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one-glo reagent promega e6120 (Promega)

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Promega one-glo reagent promega e6120
One Glo Reagent Promega E6120, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/one-glo+reagent+e6120/one+glotm+luciferase+assay+system/us12351630-807-24-26
Average 90 stars, based on 1 article reviews

one-glo reagent promega e6120 - by Bioz Stars, 2026-10

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Article Title: Bivalent SARS-CoV-2 mRNA vaccines increase breadth of neutralization and protect against the BA.5 Omicron variant
Article Snippet: At 18 h post infection, an equal volume of One-Glo reagent (Promega; E6120) was added to culture medium for readout using BMG PHERastar-FSX plate reader.

Article Title: Regulation of gene transcription by thyroid hormone receptor β agonists in clinical development for the treatment of non-alcoholic steatohepatitis (NASH)
Article Snippet: After approximately 18 to 24 hrs, the culture plates were equilibrated to RT, 30 μL ONE-Glo reagent (Promega, E6120) was added to each well, and luminescence was measured on an EnSpire plate reader (Perkin Elmer).

Article Title: Viral evolution prediction identifies broadly neutralizing antibodies to existing and prospective SARS-CoV-2 variants.
Article Snippet: At 18 h after infection, an equal volume of One-Glo reagent (E6120; Promega) was Nature Microbiology Article https://doi.org/10.1038/s41564-025-02030-7 added to the culture medium for read-out using a BMG PHERastar-FSX plate reader.

Article Title: Bivalent SARS-CoV-2 mRNA vaccines increase breadth of neutralization and protect against the BA.5 Omicron variant in mice.
Article Snippet: The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in the Omicron lineage has resulted in diminished Coronavirus Disease 2019 (COVID-19) vaccine efficacy and persistent transmission.. In this study, we evaluated the immunogenicity and protective efficacy of two, recently authorized, bivalent COVID-19 vaccines that contain two mRNAs encoding Wuhan-1 and either BA.1 (mRNA-1273.214) or BA.4/5 (mRNA-1273.222) spike proteins.. As a primary two-dose immunization series in mice, both bivalent vaccines induced greater neutralizing antibody responses against Omicron variants than the parental, monovalent mRNA-1273 vaccine.

Article Title: Distinct sensitivities to SARS-CoV-2 variants in vaccinated humans and mice.
Article Snippet: At 18 hr post infection, equal volume of One-Glo reagent (Promega; E6120) was added to culture medium for readout using BMG PHERastar-FS plate reader.

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Tumor localized FAP + CAFs inhibit CAR T cell intra-tumoral infiltration and anti-tumor cytotoxicity (A) Schematic illustrating T cell infiltration assay in tumor spheroids plated with or without CAFs. (B) Graph depicting flow cytometry quantitation of T cells infiltrated per tumor spheroids with or without CAFs, represented as percentage of T cell input. Bars show means ± SD, n = 3; p values determined by Student t test (two-tailed, unpaired). ns, not significant, ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001. (C) Schematic of TRAC KO ML CAR T cell cytotoxicity assay against tumor spheroids of TNBC cell line MDA-MB-231-Luc alone or co-cultured with TNBC-derived CAFs. (D) Bar graph representing percentage MDA-MB-231-Luc tumor cell survival post cytotoxicity assay outlined in (C), at Effector:Target ratio = 5:1. Bars show means ± SD, n = 3; p values determined by Student t test (two-tailed, unpaired). ns, not significant, ∗ p ≤ 0.05, ∗∗ p ≤ 0.01. (E) Immunohistochemistry for detection of human FAP protein in tissue microarray of patient samples from different cancers. (F) Heatmap depicting percentage positive area stained for human FAP protein in immunohistochemical analysis of healthy human tissue microarray.

Journal: Molecular Therapy

Article Title: TALEN-edited allogeneic inducible dual CAR T cells enable effective targeting of solid tumors while mitigating off-tumor toxicity

doi: 10.1016/j.ymthe.2024.08.018

Figure Lengend Snippet: Tumor localized FAP + CAFs inhibit CAR T cell intra-tumoral infiltration and anti-tumor cytotoxicity (A) Schematic illustrating T cell infiltration assay in tumor spheroids plated with or without CAFs. (B) Graph depicting flow cytometry quantitation of T cells infiltrated per tumor spheroids with or without CAFs, represented as percentage of T cell input. Bars show means ± SD, n = 3; p values determined by Student t test (two-tailed, unpaired). ns, not significant, ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001. (C) Schematic of TRAC KO ML CAR T cell cytotoxicity assay against tumor spheroids of TNBC cell line MDA-MB-231-Luc alone or co-cultured with TNBC-derived CAFs. (D) Bar graph representing percentage MDA-MB-231-Luc tumor cell survival post cytotoxicity assay outlined in (C), at Effector:Target ratio = 5:1. Bars show means ± SD, n = 3; p values determined by Student t test (two-tailed, unpaired). ns, not significant, ∗ p ≤ 0.05, ∗∗ p ≤ 0.01. (E) Immunohistochemistry for detection of human FAP protein in tissue microarray of patient samples from different cancers. (F) Heatmap depicting percentage positive area stained for human FAP protein in immunohistochemical analysis of healthy human tissue microarray.

Article Snippet: On the day of measuring cytotoxicity, ONE-Glo reagent was prepared following the manufacturer’s instructions (Promega, #E6120).

Techniques: Flow Cytometry, Quantitation Assay, Two Tailed Test, Cytotoxicity Assay, Cell Culture, Derivative Assay, Immunohistochemistry, Microarray, Staining, Immunohistochemical staining

FAP CAR activation-dependent stringent regulation of ML CAR expression and activity in multiplex engineered FAPCAR_ΔPD1 ML CAR UT cells (A) Flow cytometry plots depicting FAP CAR and ML CAR expression in indicated UT cell groups, with or without human FAP (FAP) protein-mediated FAP CAR activation for 48 h. (B) Graph representing quantitation of percentage of ML CAR-positive viable T cells, as determined in (A). Bars show the means ± SD, n = 3 donors; p values determined by Student t test (two-tailed, unpaired). ns, not significant, ∗ p ≤ 0.05. (C) Graph depicting kinetics of FAP CAR and ML CAR expression following FAP protein-mediated activation of FAP CAR on FAPCAR_ΔPD1 ML CAR UT cells at 0 h. Each data point represents mean ± SD, n = 3 donors. (D) Graph representing time course of FAP CAR and MLCAR expression upon FAP CAR stimulation and withdrawal of stimulus (FAP protein) from FAPCAR_ΔPD1 ML CAR UT cells. Each data point represents mean ± SD, n = 3 donors. (E) Schematic for assessing ML CAR cytotoxicity of indicated UT cells against ML + FAP − NCI-H226-LUC tumor cells upon FAP CAR stimulation with FAP for 3 days, and subsequent withdrawal of stimulus (FAP protein) for 4 days. (F) Bar graph representing percentage ML + FAP − NCI-H226-LUC tumor cell killing at different time points defined in (E). Cytotoxicity was measured 24 h post incubation of UT cells taken from indicated time points in (E) with target cells at Effector:Target ratio = 1:1. Bars show the means ± SD, n = 2 independent experiments; p values determined by Student t test (two-tailed, unpaired). ns, not significant, ∗ p ≤ 0.05.

Journal: Molecular Therapy

Article Title: TALEN-edited allogeneic inducible dual CAR T cells enable effective targeting of solid tumors while mitigating off-tumor toxicity

doi: 10.1016/j.ymthe.2024.08.018

Figure Lengend Snippet: FAP CAR activation-dependent stringent regulation of ML CAR expression and activity in multiplex engineered FAPCAR_ΔPD1 ML CAR UT cells (A) Flow cytometry plots depicting FAP CAR and ML CAR expression in indicated UT cell groups, with or without human FAP (FAP) protein-mediated FAP CAR activation for 48 h. (B) Graph representing quantitation of percentage of ML CAR-positive viable T cells, as determined in (A). Bars show the means ± SD, n = 3 donors; p values determined by Student t test (two-tailed, unpaired). ns, not significant, ∗ p ≤ 0.05. (C) Graph depicting kinetics of FAP CAR and ML CAR expression following FAP protein-mediated activation of FAP CAR on FAPCAR_ΔPD1 ML CAR UT cells at 0 h. Each data point represents mean ± SD, n = 3 donors. (D) Graph representing time course of FAP CAR and MLCAR expression upon FAP CAR stimulation and withdrawal of stimulus (FAP protein) from FAPCAR_ΔPD1 ML CAR UT cells. Each data point represents mean ± SD, n = 3 donors. (E) Schematic for assessing ML CAR cytotoxicity of indicated UT cells against ML + FAP − NCI-H226-LUC tumor cells upon FAP CAR stimulation with FAP for 3 days, and subsequent withdrawal of stimulus (FAP protein) for 4 days. (F) Bar graph representing percentage ML + FAP − NCI-H226-LUC tumor cell killing at different time points defined in (E). Cytotoxicity was measured 24 h post incubation of UT cells taken from indicated time points in (E) with target cells at Effector:Target ratio = 1:1. Bars show the means ± SD, n = 2 independent experiments; p values determined by Student t test (two-tailed, unpaired). ns, not significant, ∗ p ≤ 0.05.

Article Snippet: On the day of measuring cytotoxicity, ONE-Glo reagent was prepared following the manufacturer’s instructions (Promega, #E6120).

Techniques: Activation Assay, Expressing, Activity Assay, Multiplex Assay, Flow Cytometry, Quantitation Assay, Two Tailed Test, Incubation

FAPCAR_ΔPD1 ML CAR UT cells display efficient FAP CAR activation-dependent dual CAR tumor killing in vitro (A) Pictorial representation of strategy designed to test activity and sensitivity of the IF/THEN logic gate in FAPCAR_ΔPD1 ML CAR UT cells. As depicted, tumor cells with no FAP expression should not induce FAP CAR-mediated ML CAR expression and should therefore survive, despite being ML positive. On the other hand, ML + FAP + double-positive cells should induce FAP CAR-mediated ML CAR expression, resulting in dual CAR activity and enhanced killing of target cells. (B) Schematic of engineered UT cell cytotoxicity assay against tumor spheroids NCI-H226-Luc cells with or without varying proportions of FAP + cells, co-incubated for 72 h at E:T = 2.5:1. (C) Heatmap representing percentage target tumor cell cytotoxicity when treated with indicated UT groups, as outlined in (B). Brackets indicate statistical comparison of cytotoxic activity between different target cell groups, n = 2 independent experiments, two donors per experiment, three technical replicates per donor per experiment; p values determined by Student t test (two-tailed, unpaired). ns, not significant, ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001. (D) Schematic of engineered UT cell cytotoxicity assay against 3D spheroids of TNBC cell line HCC70-NanoLuc alone or co-cultured with TNBC-derived CAFs at 1:2 ratio. Effector and target spheroids co-incubated for 72 h at 5:1 ratio. (E) Bar graph representing percentage of HCC70-GFP tumor cell cytotoxicity post cytotoxicity assay outlined in (D). Data representative of n = 2 independent experiments, two donors per experiment, three technical replicates per donor per experiment. Bars show the means ± SD; p values determined by Student t test (two-tailed, unpaired). ns, not significant, ∗ p ≤ 0.05, ∗∗ p ≤ 0.01. (F) Bar graph representing IFNγ release in cytotoxicity assay outlined in (D), as measured by ELISA in co-culture supernatant on day 6. Data representative of n = 2 independent experiments, two donors per experiment, three technical replicates per donor per experiment. Bars show the means ± SD; p values determined by Student t test (two-tailed, unpaired). ns, not significant, ∗ p ≤ 0.05.

Journal: Molecular Therapy

Article Title: TALEN-edited allogeneic inducible dual CAR T cells enable effective targeting of solid tumors while mitigating off-tumor toxicity

doi: 10.1016/j.ymthe.2024.08.018

Figure Lengend Snippet: FAPCAR_ΔPD1 ML CAR UT cells display efficient FAP CAR activation-dependent dual CAR tumor killing in vitro (A) Pictorial representation of strategy designed to test activity and sensitivity of the IF/THEN logic gate in FAPCAR_ΔPD1 ML CAR UT cells. As depicted, tumor cells with no FAP expression should not induce FAP CAR-mediated ML CAR expression and should therefore survive, despite being ML positive. On the other hand, ML + FAP + double-positive cells should induce FAP CAR-mediated ML CAR expression, resulting in dual CAR activity and enhanced killing of target cells. (B) Schematic of engineered UT cell cytotoxicity assay against tumor spheroids NCI-H226-Luc cells with or without varying proportions of FAP + cells, co-incubated for 72 h at E:T = 2.5:1. (C) Heatmap representing percentage target tumor cell cytotoxicity when treated with indicated UT groups, as outlined in (B). Brackets indicate statistical comparison of cytotoxic activity between different target cell groups, n = 2 independent experiments, two donors per experiment, three technical replicates per donor per experiment; p values determined by Student t test (two-tailed, unpaired). ns, not significant, ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001. (D) Schematic of engineered UT cell cytotoxicity assay against 3D spheroids of TNBC cell line HCC70-NanoLuc alone or co-cultured with TNBC-derived CAFs at 1:2 ratio. Effector and target spheroids co-incubated for 72 h at 5:1 ratio. (E) Bar graph representing percentage of HCC70-GFP tumor cell cytotoxicity post cytotoxicity assay outlined in (D). Data representative of n = 2 independent experiments, two donors per experiment, three technical replicates per donor per experiment. Bars show the means ± SD; p values determined by Student t test (two-tailed, unpaired). ns, not significant, ∗ p ≤ 0.05, ∗∗ p ≤ 0.01. (F) Bar graph representing IFNγ release in cytotoxicity assay outlined in (D), as measured by ELISA in co-culture supernatant on day 6. Data representative of n = 2 independent experiments, two donors per experiment, three technical replicates per donor per experiment. Bars show the means ± SD; p values determined by Student t test (two-tailed, unpaired). ns, not significant, ∗ p ≤ 0.05.

Article Snippet: On the day of measuring cytotoxicity, ONE-Glo reagent was prepared following the manufacturer’s instructions (Promega, #E6120).

Techniques: Activation Assay, In Vitro, Activity Assay, Expressing, Cytotoxicity Assay, Incubation, Comparison, Two Tailed Test, Cell Culture, Derivative Assay, Enzyme-linked Immunosorbent Assay, Co-Culture Assay

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